The Chemistry of Photoresponsive Systems team is pleased to announce that the eFM dyes — enhanced FM dyes — are now commercially available from Cytoskeleton, Inc.
These next-generation fluorescent probes enable rapid, efficient, and fluorogenic labeling of the plasma membrane in bothanimal and plant cells. Developed through the rational optimization of classical FM dyes, they retain the key advantagesof the FM dye family, including high photostability and large Stokes shifts, while offering improved performance: enhanced plasma membrane specificity, reduced background, lower endosomal internalization, and better compatibility with multicolor imaging.
Two probes are currently available: eFM488 and eFM560. They have been validated for various microscopy approaches, including confocal imaging and STED microscopy, in live and fixed cells, tissues, plant cells, and extracellular vesicles.
This commercialization represents an important step in the transfer of fluorescent probes developed by the CSP team to the broader scientific community.
More information and ordering details are available from Cytoskeleton:
https://www.cytoskeleton.com/mechanisms-and-characteristics-of-enhanced-fluorogenic-plasma-membrane-probes
Associated publication:
Collot, M.; Boutant, E.; Fam, K. T.; Danglot, L.; Klymchenko, A. S. Molecular Tuning of Styryl Dyes Leads to Versatile and Efficient Plasma Membrane Probes for Cell and Tissue Imaging. Bioconjugate Chemistry 2020, 31 (3), 875–883.
